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| Registros recuperados : 40 | |
| 1. |  | FERNÁNDEZ ,S.; FRAGA, M.; CASTELLS, M.; COLINA, R.; ZUNINO,P. Effect of the administration of Lactobacillus spp. strains on neonatal diarrhoea, immune parameters and pathogen abundance in pre-weaned calves. Beneficial Microbes, 1 September 2020, Volume 11, Issue 5, Pages 477-488. Doi: https://doi.org/10.3920/BM2019.0167| Biblioteca(s): INIA La Estanzuela. |
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| 3. | | RUBIO, L.; BERTALMIO, A.; RIVAS, F.; COLINA, R.; BENÍTEZ, M. J.; MAESO, D. Caracterización molecular y biológica de aislamientos de campo de CTV de Uruguay y su potencial en la protección cruzada. In: INIA (Instituto Nacional de Investigación Agropecuaria); Universidad de la República (UdelaR), Facultad de Agronomía. Simposio Nacional, 4. y Congreso Latinoamericano, 1. ?Investigación y Desarrollo Tecnológico en Citrus?, 3-5 noviembre 2014, Montevideo (Uruguay). p. 1-4.| Biblioteca(s): INIA Las Brujas. |
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| 4. | | BENÍTEZ, M. J.; BERTALMIO, A.; RUBIO, L.; RIVAS, F.; MAESO, D.; COLINA, R. Caracterización molecular y filodinámica del virus de la tristeza de los cítricos. In: INIA (Instituto Nacional de Investigación Agropecuaria); Universidad de la República (UdelaR), Facultad de Agronomía. Simposio Nacional, 4. y Congreso Latinoamericano, 1. ?Investigación y Desarrollo Tecnológico en Citrus?, 3-5 noviembre 2014, Montevideo (Uruguay). p. 1-4.| Biblioteca(s): INIA Las Brujas. |
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| 5. | | RUBIO, L.; HERNÁNDEZ, L.; BENÍTEZ, M. J.; ARRUABARRENA, A.; RIVAS, F.; COLINA, R.; MAESO, D. Biological and molecular characterization of Uruguayan citrus tristeza virus field isolates. Journal of Plant Pathology, 2019, volume 101, Issue 1, pages 97-105. Article history: Received 06 June 2018 // Accepted 29 July 2018 // First Online 13 August 2018 // Published 15 February 2019.
Acknowledgements: This research was funded by Instituto Nacional de Investigación Agropecuaria, Uruguay...| Biblioteca(s): INIA Las Brujas. |
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| 7. | | CAFFARENA, D.; FRAGA, M.; CASTELLS, M.; COLINA, R.; SCHILD, C.; RIET-CORREA, F.; GIANNITTI, F. Detección de agentes causales de diarrea neonatal bovina en 2 tambos de Colonia, Uruguay. In: Jornadas Uruguayas de Buiatría, 44., 2016, Paysandú, UY.; Gianneechini, E.; Elizondo, V. (Ed.).Paysandú: Centro Médico Veterinario de Paysandú/Sociedad Uruguaya de Buiatría, 2016. p. 153-156.| Biblioteca(s): INIA La Estanzuela. |
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| 8. | | BENÍTEZ, M.J.; BERTONI, E.; BERTALMIO, A.; RUBIO, L.; RIVAS, C.; MAESO, D.; COLINA, R. Detección y caracterización de virus y viroides en la Citricultura del Uruguay: aportes sobre el nuevo linaje de CTV en Sudamérica y su repercusión en la protección cruzada. In: INIA (Instituto Nacional de Investigación Agropecuaria); Programa Nacional Producción Citrícola. Resultados de investigación en Citricultura: Genética, Sanidad, Productividad. Salto (Uruguay): INIA, 2015. p. 107-112 (Serie Actividades de Difusión; 752).| Biblioteca(s): INIA Las Brujas. |
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| 10. | | BOK, M.; VEGA, C.G.; CASTELLS, M.; COLINA, R.; WIGDOROVITZ, A.; PARREÑO, V. Development of an IgY-based treatment to control bovine coronavirus diarrhea in dairy calves. Viruses, 2023, volume 15, issue 3, article 708. OPEN ACCESS. doi: https://doi.org/10.3390/v15030708 Article history: Received 6 February 2023; Revised 5 March 2023; Accepted 6 March 2023; Published 9 March 2023. -- Academic Editor: Tomomi Takano. -- Correspondence author: Bok, M.; INCUINTA, Virology Institute and Technology Innovations,...| Biblioteca(s): INIA Las Brujas. |
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| 11. | | DONCEL, B.; CASTELLS, M.; MAYA, L.; FRAGA, M.; UZAL, F.A; COLINA, R.; GIANNITTI, F. Encefalitis asociada a astrovirus bovino neurotrópico, ¿una enfermedad subdiagnosticada en Sudamérica?.[Neurotropic bovine astrovirus-associated encephalitis: An underdiagnosed disease in South America?]. Revista Argentina de Microbiologia, Volume 54, Pages 100-105, 2022. OPEN ACCESS. Doi: https://doi.org/10.1016/j.ram.2021.01.00 Article history: Recibido el 15 de julio de 2020/ Aaceptado el 24 de enero de 2021./Disponible en Internet el 18 junio 2021. Autor para correspondencia: Correo electrónico: fgiannitti@inia.org.uy (F. Giannitti). Este trabajo fue...| Biblioteca(s): INIA La Estanzuela. |
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| 12. | | MAISONNAVE, J.; ROSSI, S.; ACOSTA, D.; HERNÁNDEZ, S.; TERZAGHI, A.; PIGNATARO, G.; COLINA, R. Evaluación de técnicas serológicas para el diagnóstico de la fasciolosis animal In: INIA (INSTITUTO NACIONAL DE INVESTIGACIÓN AGROPECUARIA); FPTA (FONDO DE PROMOCIÓN DE TECNOLOGÍA AGROPECUARIA). Avances de investigación en sanidad animal. Montevideo (Uruguay): INIA, 2000. p. 39-51 (Serie FPTA-INIA ; 01) Proyecto FPTA 25. Período de ejecución: Oct.,1991-May.,1995| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
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| 13. | | BENÍTEZ-GALEANO, M.J.; RUBIO, L.; BERTALMIO, A.; MAESO, D.; RIVAS, F.; COLINA, R. Phylogenetic studies of the three RNA silencing suppressor genes of south american ctv isolates reveal the circulation of a novel genetic lineage. Viruses, 2015, v.7, no.7, p.4152-4168. OPEN ACCESS. Article history: Received: 26 May 2015 / Accepted: 17 July 2015 / Published: 22 July 2015.| Biblioteca(s): INIA Las Brujas. |
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| 14. | | MAESO, D.; RUBIO, L.; BENÍTEZ-GALEANO, M.J.; HERNÁNDEZ, L.; BERTALMIO, A.; ARRUABARRENA, A.; RIVAS, F.; COLINA, R. Aportes al conocimiento de Citrus tristeza virus en Uruguay. [resumen] In: INIA (Instituto Nacional de Investigación Agropecuaria); INIA Las Brujas; Biotecnología. Jornada de Agrobiotecnología, XI. Encuentro Nacional de REDBIO, III. Jornada técnica. Las Brujas, Canelones (UY): INIA, 2018. p. 12 (Serie Actividades de Difusión; 786)| Biblioteca(s): INIA Las Brujas. |
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| 15. | | MAYA, L.; PUENTES. R.; RIET-CORREA, F.; GIANNITTI, F.; RIVERO, R.; GINNNEECHINI, E.; FURTADO FLORES, E.; COLINA, R. Caracterización genética del virus de la diarrea viral bovina en Uruguay. In: Jornadas Uruguayas de Buiatría, 46., 2018, Paysandú, Uy., Matto, C.; Delpiazzo, R.(Ed.). Trabajos presentados. Paysandú: Centro Médico Veterinario de Paysandú/Sociedad Uruguaya de Buiatría, 2018. p. 183-185.| Biblioteca(s): INIA La Estanzuela. |
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| 16. | | CASTELLS, M.; CAFFARENA, D.; CASAUX, L.; SCHILD, C.; GIANNITTI, F.; MIÑO, S.; RIET-CORREA, F.; PARREÑO, V.; COLINA, R. Determinación de la prevalencia y variabilidad genética de rotavirus: coronavirus, torovirus, norovirus y astrovirus. In: Jornadas Uruguayas de Buiatría, 46., 2018, Paysandú, Uy., Matto, C.; Delpiazzo, R.(Ed.). Trabajos presentados. Paysandú: Centro Médico Veterinario de Paysandú/Sociedad Uruguaya de Buiatría, 2018. p. 230-233.| Biblioteca(s): INIA La Estanzuela. |
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| 17. | | CASTELLS, M.; SCHILD, C.; CAFFARENA, D.; GIANNITTI, F.; FRAGA, M.; RIET-CORREA, F.; VICTORIA, M.; COLINA, R. Drinking water: a potential vehicle for rotavirus transmission in neonate calves. In: LATIN AMERICAN SYMPOSIUM OF ENVIRONMENTAL VIROLOGY, 3a.,Salta, Argentina, 2016.| Biblioteca(s): INIA La Estanzuela. |
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| 18. | | GIANNITTI, F.; CAFFARENA, D.; PESAVENTO, P.; UZAL, F.A.; MAYA, L.; FRAGA, M.; COLINA, R.; CASTELLS, M. The first case of bovine astrovirus-associated encephalitis in the southern hemisphere (Uruguay), uncovers evidence of viral introduction to the Americas from Europe. Frontiers in Microbiology, volumen 10, Article 1240, 04 June 2019. [OPEN ACCESS].Doi: https://doi.org/10.3389/fmicb.2019.01240 Article history: Received: 22 March 2019//Accepted: 17 May 2019//Published: 04 June 2019. This work was funded by Grants PL-015 N-15156 from INIA and 158 from the ?Programa de Iniciación a la Investigación 2017? from ?Comisión Sectorial...| Biblioteca(s): INIA La Estanzuela. |
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| 19. | | HERNÁNDEZ-RODRÍGUEZ, L.; BERTALMIO, A.; ARRUABARRENA, A.; RUBIO, L.; RIVAS, F.; BENÍTEZ-GALEANO, M.J.; COLINA, R.; MAESO, D. First Report of the Citrus tristeza virus Trifoliate Resistance-Breaking (RB) Genotype in "Newhall" Sweet Orange in South America Plant Disease, 2017, v.101 (6), p.1063. (Abstract) DISEASE NOTES: Posted online on 30 Mar 2017.| Biblioteca(s): INIA Las Brujas. |
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| 20. | | MAYA, L.; PUENTES, R.; REOLÓN, E.; ACUÑA, P.; RIET-CORREA, F.; RIVERO, R.; CRISTINA, J.; COLINA, R. Molecular diversity of bovine viral diarrhea virus in Uruguay. Archives of Virology, 2016, v.161, no.3, p. 529-535. Article history: Received: 1 July 2015 / Accepted: 14 November 2015 / Published online: 23 November 2015| Biblioteca(s): INIA Las Brujas. |
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| Registros recuperados : 40 | |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
19/04/2023 |
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Actualizado : |
19/04/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
BOK, M.; VEGA, C.G.; CASTELLS, M.; COLINA, R.; WIGDOROVITZ, A.; PARREÑO, V. |
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Afiliación : |
MARINA BOK, INCUINTA, Virology Institute and Technology Innovations, National Institute of Agricultural Technology, Buenos Aires, 1686, Argentina; CONICET, Buenos Aires, C1425FQB, Argentina; Bioinnovo S.A, Buenos Aires, 1686, Argentina; CELINA G. VEGA, INCUINTA, Virology Institute and Technology Innovations, National Institute of Agricultural Technology, Buenos Aires, 1686, Argentina; CONICET, Buenos Aires, C1425FQB, Argentina; Bioinnovo S.A, Buenos Aires, 1686, Argentina; MATÍAS CASTELLS BAUER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Molecular Virology Laboratory, CENUR North Litoral, Salto University Centre, University of the Republic, Salto, 50000, Uruguay; RODNEY COLINA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Molecular Virology Laboratory, CENUR North Litoral, Salto University Centre, University of the Republic, Salto, 50000, Uruguay; ANDRÉS WIGDOROVITZ, INCUINTA, Virology Institute and Technology Innovations, National Institute of Agricultural Technology, Buenos Aires, 1686, Argentina; CONICET, Buenos Aires, C1425FQB, Argentina; Bioinnovo S.A, Buenos Aires, 1686, Argentina; VIVIANA PARREÑO, INCUINTA, Virology Institute and Technology Innovations, National Institute of Agricultural Technology, Buenos Aires, 1686, Argentina; CONICET, Buenos Aires, C1425FQB, Argentina; Bioinnovo S.A, Buenos Aires, 1686, Argentina. |
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Título : |
Development of an IgY-based treatment to control bovine coronavirus diarrhea in dairy calves. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Viruses, 2023, volume 15, issue 3, article 708. OPEN ACCESS. doi: https://doi.org/10.3390/v15030708 |
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ISSN : |
1999-4915 |
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DOI : |
10.3390/v15030708 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 6 February 2023; Revised 5 March 2023; Accepted 6 March 2023; Published 9 March 2023. -- Academic Editor: Tomomi Takano. -- Correspondence author: Bok, M.; INCUINTA, Virology Institute and Technology Innovations, National Institute of Agricultural Technology, Buenos Aires 1686, Argentina; email:bok.marina@inta.gob.ar ; Parreño, V.; INCUINTA, Virology Institute and Technology Innovations, National Institute of Agricultural Technology, Buenos Aires, Argentina; email:parreno.viviana@inta.gob.ar -- This article belongs to the Special Issue Animal Coronaviruses: Infection, Prevention, and Antivirals https://www.mdpi.com/journal/viruses/special_issues/5XDFOYZ724 ) -- Funding: This work was supported by The National Institute of Agricultural Technology (INTA), ANPCyT from the Argentinean Agricultural Ministry (PICT Start Up 2010-1532), and Vetanco S.A. (CVT 20794). -- |
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Contenido : |
Bovine Coronavirus (BCoV) is a major pathogen associated with neonatal calf diarrhea. Standard practice dictates that to prevent BCoV diarrhea, dams should be immunized in the last stage of pregnancy to increase BCoV-specific antibody (Ab) titers in serum and colostrum. For the prevention to be effective, calves need to suck maternal colostrum within the first six to twelve hours of life before gut closure to ensure a good level of passive immunity. The high rate of maternal Ab transfer failure resulting from this process posed the need to develop alternative local passive immunity strategies to strengthen the prevention and treatment of BCoV diarrhea. Immunoglobulin Y technology represents a promising tool to address this gap. In this study, 200 laying hens were immunized with BCoV to obtain spray-dried egg powder enriched in specific IgY Abs to BCoV on a large production scale. To ensure batch-to-batch product consistency, a potency assay was statistically validated. With a sample size of 241, the BCoV-specific IgY ELISA showed a sensitivity and specificity of 97.7% and 98.2%, respectively. ELISA IgY Abs to BCoV correlated with virus-neutralizing Ab titers (Pearson correlation, R2 = 0.92, p < 0.001). Most importantly, a pilot efficacy study in newborn calves showed a significant delay and shorter duration of BCoV-associated diarrhea and shedding in IgY-treated colostrum-deprived calves. Calves were treated with milk supplemented with egg powder (final IgY Ab titer to BCoV ELISA = 512; VN = 32) for 14 days as a passive treatment before a challenge with BCoV and were compared to calves fed milk with no supplementation. This is the first study with proof of efficacy of a product based on egg powder manufactured at a scale that successfully prevents BCoV-associated neonatal calf diarrhea. © 2023 by the authors. MenosBovine Coronavirus (BCoV) is a major pathogen associated with neonatal calf diarrhea. Standard practice dictates that to prevent BCoV diarrhea, dams should be immunized in the last stage of pregnancy to increase BCoV-specific antibody (Ab) titers in serum and colostrum. For the prevention to be effective, calves need to suck maternal colostrum within the first six to twelve hours of life before gut closure to ensure a good level of passive immunity. The high rate of maternal Ab transfer failure resulting from this process posed the need to develop alternative local passive immunity strategies to strengthen the prevention and treatment of BCoV diarrhea. Immunoglobulin Y technology represents a promising tool to address this gap. In this study, 200 laying hens were immunized with BCoV to obtain spray-dried egg powder enriched in specific IgY Abs to BCoV on a large production scale. To ensure batch-to-batch product consistency, a potency assay was statistically validated. With a sample size of 241, the BCoV-specific IgY ELISA showed a sensitivity and specificity of 97.7% and 98.2%, respectively. ELISA IgY Abs to BCoV correlated with virus-neutralizing Ab titers (Pearson correlation, R2 = 0.92, p < 0.001). Most importantly, a pilot efficacy study in newborn calves showed a significant delay and shorter duration of BCoV-associated diarrhea and shedding in IgY-treated colostrum-deprived calves. Calves were treated with milk supplemented with egg powder (final IgY Ab titer to BCoV ... Presentar Todo |
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Palabras claves : |
Bovine coronavirus; Development; ELISA validation; IgY antibodies; Neonatal calf diarrhea. |
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Asunto categoría : |
L20 Ecología animal |
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URL : |
https://www.mdpi.com/1999-4915/15/3/708/pdf
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Marc : |
LEADER 03567naa a2200277 a 4500
001 1064043
005 2023-04-19
008 2023 bl uuuu u00u1 u #d
022 $a1999-4915
024 7 $a10.3390/v15030708$2DOI
100 1 $aBOK, M.
245 $aDevelopment of an IgY-based treatment to control bovine coronavirus diarrhea in dairy calves.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 6 February 2023; Revised 5 March 2023; Accepted 6 March 2023; Published 9 March 2023. -- Academic Editor: Tomomi Takano. -- Correspondence author: Bok, M.; INCUINTA, Virology Institute and Technology Innovations, National Institute of Agricultural Technology, Buenos Aires 1686, Argentina; email:bok.marina@inta.gob.ar ; Parreño, V.; INCUINTA, Virology Institute and Technology Innovations, National Institute of Agricultural Technology, Buenos Aires, Argentina; email:parreno.viviana@inta.gob.ar -- This article belongs to the Special Issue Animal Coronaviruses: Infection, Prevention, and Antivirals https://www.mdpi.com/journal/viruses/special_issues/5XDFOYZ724 ) -- Funding: This work was supported by The National Institute of Agricultural Technology (INTA), ANPCyT from the Argentinean Agricultural Ministry (PICT Start Up 2010-1532), and Vetanco S.A. (CVT 20794). --
520 $aBovine Coronavirus (BCoV) is a major pathogen associated with neonatal calf diarrhea. Standard practice dictates that to prevent BCoV diarrhea, dams should be immunized in the last stage of pregnancy to increase BCoV-specific antibody (Ab) titers in serum and colostrum. For the prevention to be effective, calves need to suck maternal colostrum within the first six to twelve hours of life before gut closure to ensure a good level of passive immunity. The high rate of maternal Ab transfer failure resulting from this process posed the need to develop alternative local passive immunity strategies to strengthen the prevention and treatment of BCoV diarrhea. Immunoglobulin Y technology represents a promising tool to address this gap. In this study, 200 laying hens were immunized with BCoV to obtain spray-dried egg powder enriched in specific IgY Abs to BCoV on a large production scale. To ensure batch-to-batch product consistency, a potency assay was statistically validated. With a sample size of 241, the BCoV-specific IgY ELISA showed a sensitivity and specificity of 97.7% and 98.2%, respectively. ELISA IgY Abs to BCoV correlated with virus-neutralizing Ab titers (Pearson correlation, R2 = 0.92, p < 0.001). Most importantly, a pilot efficacy study in newborn calves showed a significant delay and shorter duration of BCoV-associated diarrhea and shedding in IgY-treated colostrum-deprived calves. Calves were treated with milk supplemented with egg powder (final IgY Ab titer to BCoV ELISA = 512; VN = 32) for 14 days as a passive treatment before a challenge with BCoV and were compared to calves fed milk with no supplementation. This is the first study with proof of efficacy of a product based on egg powder manufactured at a scale that successfully prevents BCoV-associated neonatal calf diarrhea. © 2023 by the authors.
653 $aBovine coronavirus
653 $aDevelopment
653 $aELISA validation
653 $aIgY antibodies
653 $aNeonatal calf diarrhea
700 1 $aVEGA, C.G.
700 1 $aCASTELLS, M.
700 1 $aCOLINA, R.
700 1 $aWIGDOROVITZ, A.
700 1 $aPARREÑO, V.
773 $tViruses, 2023, volume 15, issue 3, article 708. OPEN ACCESS. doi: https://doi.org/10.3390/v15030708
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